Structural basis for the pore-forming activity of a complement-like toxin

Abstract

Pore-forming proteins comprise a highly diverse group of proteins exemplified by the membrane attack complex/ perforin (MACPF), cholesterol-dependent cytolysin (CDC), and gasdermin superfamilies, which all form gigantic pores (>150 angstroms). A recently found family of pore-forming toxins, called CDC-like proteins (CDCLs), are wide-spread in gut microbes and are a prevalent means of antibacterial antagonism. However, the structural aspects of how CDCLs assemble a pore remain a mystery. Here, we report the crystal structure of a proteolytically activated CDCL and cryo–electron microscopy structures of a prepore-like intermediate and a transmembrane pore providing detailed snapshots across th..